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#1486174 - 01/09/09 05:03 PM Re: Not Mothers in micro-culture [Re: Loki]
RRog Carpal Tunnel Registered: 08/26/08 Posts: 2326	It's all off the wall. The info online, the availability of good equipment (electrophoresis) and specialty bio-products like ampicillin, plasmid vectors, cultures. Freaking crazy exciting. _________________________ I am not an Expert. I have so many posts because I ask a lot of questions.
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#1486178 - 01/09/09 05:24 PM list of Cannaboids [Re: RRog]
Loki Old hand Registered: 03/31/08 Posts: 1165 Loc: Laughing Skull (US)	Just wanted to see how this list of Cannaboids looks... Cannabigerol-type (CBG) Cannabigerol [(E)-CBG-C5] Cannabigerol monomethyl ether[(E)-CBGM-C5 A] Cannabinerolic acid A [(Z)-CBGA-C5 A] Cannabigerovarin [(E)-CBGV-C3] Cannabigerolic acid A [(E)-CBGA-C5 A] Cannabigerolic acid A monomethyl ether [(E)-CBGAM-C5 A] Cannabigerovarinic acid A [(E)-CBGVA-C3 A] Cannabichromene-type (CBC) (±)-Cannabichromene[CBC-C5] (±)-Cannabichromenic acid A [CBCA-C5 A] (±)-Cannabivarichromene, (±)-Cannabichromevarin [CBCV-C3] (±)-Cannabichromevarinic acid A[CBCVA-C3 A] Cannabidiol-type (CBD) (−)-Cannabidiol[CBD-C5] Cannabidiol momomethyl ether [CBDM-C5] Cannabidiol-C4[CBD-C4] (−)-Cannabidivarin [CBDV-C3] Cannabidiorcol[CBD-C1] Cannabidiolic acid[CBDA-C5] Cannabidivarinic acid [CBDVA-C3] Cannabinodiol-type (CBND) Cannabinodiol[CBND-C5] Cannabinodivarin[CBND-C3] Tetrahydrocannabinol-type (THC) Δ9-Tetrahydrocannabinol [Δ9-THC-C5] Δ9-Tetrahydrocannabinol-C4[Δ9-THC-C4] Δ9-Tetrahydrocannabivarin [Δ9-THCV-C3] Δ9-Tetrahydrocannabiorcol[Δ9-THCO-C1] Δ9-Tetrahydro- cannabinolic acid A[Δ9-THCA-C5 A] Δ9-Tetrahydro- cannabinolic acid B [Δ9-THCA-C5 B] Δ9-Tetrahydro- cannabinolic acid-C4 A and/or B[Δ9-THCA-C4 A and/or B] Δ9-Tetrahydro- cannabivarinic acid A [Δ9-THCVA-C3 A] Δ9-Tetrahydro- cannabiorcolic acid A and/or B[Δ9-THCOA-C1 An and/or B] (−)-Δ8-trans-(6aR,10aR)- Δ8-Tetrahydrocannabinol [Δ8-THC-C5] (−)-Δ8-trans-(6aR,10aR)- Tetrahydrocannabinolic acid A[Δ8-THCA-C5 A] (−)-(6aS,10aR)-Δ9- Tetrahydrocannabinol[(−)-cis-Δ9-THC-C5] Cannabinol-type (CBN) Cannabinol[CBN-C5] Cannabinol-C4[CBN-C4] Cannabivarin [CBN-C3] Cannabinol-C2[CBN-C2] Cannabiorcol [CBN-C1] Cannabinolic acid A [CBNA-C5 A] Cannabinol methyl ether [CBNM-C5] Cannabitriol-type (CBT) (−)-(9R,10R)-trans- Cannabitriol [(−)-trans-CBT-C5] (+)-(9S,10S)-Cannabitriol [(+)-trans-CBT-C5] (±)-(9R,10S/9S,10R)- Cannabitriol[(±)-cis-CBT-C5] (−)-(9R,10R)-trans- 10-O-Ethyl-cannabitriol [(−)-trans-CBT-OEt-C5] (±)-(9R,10R/9S,10S)- Cannabitriol-C3 [(±)-trans-CBT-C3] 8,9-Dihydroxy-Δ6a(10a)- tetrahydrocannabinol [8,9-Di-OH-CBT-C5] Cannabidiolic acid A cannabitriol ester[CBDA-C5 9-OH-CBT-C5 ester] (−)-(6aR,9S,10S,10aR)- 9,10-Dihydroxy- hexahydrocannabinol, Cannabiripsol[Cannabiripsol-C5] (−)-6a,7,10a-Trihydroxy- Δ9-tetrahydrocannabinol[(−)-Cannabitetrol] 10-Oxo-Δ6a(10a)- tetrahydrocannabinol [OTHC] Cannabielsoin-type (CBE) (5aS,6S,9R,9aR)- Cannabielsoin[CBE-C5] (5aS,6S,9R,9aR)- C3-Cannabielsoin [CBE-C3] (5aS,6S,9R,9aR)- Cannabielsoic acid A[CBEA-C5 A] (5aS,6S,9R,9aR)- Cannabielsoic acid B [CBEA-C5 B] (5aS,6S,9R,9aR)- C3-Cannabielsoic acid B [CBEA-C3 B] Cannabiglendol-C3[OH-iso-HHCV-C3] Dehydrocannabifuran[DCBF-C5] Cannabifuran[CBF-C5] Isocannabinoids (−)-Δ7-trans-(1R,3R,6R)- Isotetrahydrocannabinol (±)-Δ7-1,2-cis- (1R,3R,6S/1S,3S,6R)- Isotetrahydro- cannabivarin (−)-Δ7-trans-(1R,3R,6R)- Isotetrahydrocannabivarin Cannabicyclol-type (CBL) (±)-(1aS,3aR,8bR,8cR)- Cannabicyclol[CBL-C5] (±)-(1aS,3aR,8bR,8cR)- Cannabicyclolic acid A [CBLA-C5 A] Cannabicyclol-type (CBL) (±)-(1aS,3aR,8bR,8cR)- Cannabicyclovarin [CBLV-C3] Cannabicitran [CBT-C5] Cannabichromanone-type (CBCN) Cannabichromanone [CBCN-C5] Cannabichromanone-C3[CBCN-C3] Cannabicoumaronone [CBCON-C5] -Loki _________________________ "Tastes like chicken..." - Ed Gein.
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#1486479 - 01/10/09 05:06 PM Re: Not Mothers in micro-culture [Re: RRog]
Loki Old hand Registered: 03/31/08 Posts: 1165 Loc: Laughing Skull (US)	Originally Posted By: RRog It's all off the wall. The info online, the availability of good equipment (electrophoresis) and specialty bio-products like ampicillin, plasmid vectors, cultures. Freaking crazy exciting. No doubt. I've been doing some research on gene promoters and its pretty amazing stuff. I've been specifically interested in light induced promoters. It might be possible to have specific genes expressed based solely on the spectrum of light. Interestingly enough, some of the promoters come from potatos. There's also this CRE/LOX technique that could be used as a sort of a gene expression toggle. You could use specific wavelengths of light to program the gene expression and then use the CRE/LOX technique to lock that in place. So, as soon as seeds or cell cultures develope a shoot with leaf tissue, you could hit it with say a range of far-red to infra-red wavelengths to turn on which ever flavors you'd like it to have.. and what kind of high. Then hit it with the wavelength that would kick in the CRE/LOX and permanently lock that in the DNA. -Loki _________________________ "Tastes like chicken..." - Ed Gein.
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#1486519 - 01/10/09 06:39 PM Re: Not Mothers in micro-culture [Re: Loki]
jawohio Old hand Registered: 12/17/07 Posts: 710 Loc: Northwest Ohio United States	I have followed this thread since its inception and must admit that you are doing some far out research that will definitely benefit the cannabis world. My applause goes out to you for doing this.....Peace and Pot _________________________ I do not grow marijuana "the led master of disaster" Organic Mafia
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#1486539 - 01/10/09 07:51 PM Re: Not Mothers in micro-culture [Re: jawohio]
Loki Old hand Registered: 03/31/08 Posts: 1165 Loc: Laughing Skull (US)	Originally Posted By: jawohio I have followed this thread since its inception and must admit that you are doing some far out research that will definitely benefit the cannabis world. My applause goes out to you for doing this.....Peace and Pot Thanks, but I'm really just standing on the shoulders of giants, as they say... But that aside, it is friggin cool... I feel like a kid in a candy store. There are so many possiblities... I don't know where to start. I was thinking that getting another plant to make smokable THC should be first, but now I'm waivering... I found out that the designer drug laws would apply, so it'd be just as illegal. It would be able to have a brief period of perfect camouflage when the cops wouldn't know what it was though... I'm finding more and more potentially valuable techniques and tools, so who knows what's possible. What would the Cannabis community benefit from most? I've got a plan for isolating and transfering a few proteins of the CBG, CBD and THC cannaboids, so now I'm researching terpene gene expression and gene promoters. It'll be a month or so before I get to the lab work. I'd love to hear what people want in a GMO plant... -Loki _________________________ "Tastes like chicken..." - Ed Gein.
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#1486549 - 01/10/09 08:45 PM

limonene synthase [Re: Loki]

Loki

Old hand

Registered: 03/31/08
Posts: 1165
Loc: Laughing Skull (US)

You ever wonder what causes that wonderful citris taste?

It's a terpene called limonene. Some plants carry and express a gene that creates this stuff... and this is what it looks like...

limonene synthase

protein sequence

Code:

     1  MQCIAFHQFASSSSLPIWSSIDNRFTPKTSITSISKPKPKLKSKSNLKSRSRSSTCYSIQ
    61  CTVVDNPSSTITNNSDRRSANYGPPIWSFDFVQSLPIQYKGESYTSRLNKLEKDVKRMLI
   121  GVENSLAQLELIDTIQRLGISYRFENEIISILKEKFTNNNDNPNPNYDLYATALQFRLLR
   181  QYGFEVPQEIFNNFKNHKTGEFKANISNDIMGALGLYEASFHGKKGESILEEARIFTTKC
   241  LKKYKLMSSSNNNNMTLISLLVNHALEMPLQWRITRSEAKWFIEEIYERKQDMNPTLLEF
   301  AKLDFNMLQSTYQEELKVLSRWWKDSKLGEKLPFVRDRLVECFLWQVGVRFEPQFSYFRI
   361  MDTKLYVLLTIIDDMHDIYGTLEELQLFTNALQRWDLKELDKLPDYMKTAFYFTYNFTNE
   421  LAFDVLQEHGFVHIEYFKKLMVELCKHHLQEAKWFYSGYKPTLQEYVENGWLSVGGQVIL
   481  MHAYFAFTNPVTKEALECLKDGHPNIVRHASIILRLADDLGTLSDELKRGDVPKSIQCYM
   541  HDTGASEDEAREHIKYLISESWKEMNNEDGNINSFFSNEFVQVCQNLGRASQFIYQYGDG
   601  HASQNNLSKERVLGLIITPIPM

DNA Sequence

Code:

     1  ATGCAATGTATTGCTTTTCATCAATTTGCTTCTTCTTCTTCTCTTCCTATTTGGTCTTCT
    61  ATTGATAATCGTTTTACTCCTAAAACTTCTATTACTTCTATTTCTAAACCTAAACCTAAA
   121  CTTAAATCTAAATCTAATCTTAAATCTCGTTCTCGTTCTTCTACTTGTTATTCTATTCAA
   181  TGTACTGTTGTTGATAATCCTTCTTCTACTATTACTAATAATTCTGATCGTCGTTCTGCT
   241  AATTATGGTCCTCCTATTTGGTCTTTTGATTTTGTTCAATCTCTTCCTATTCAATATAAA
   301  GGTGAATCTTATACTTCTCGTCTTAATAAACTTGAAAAAGATGTTAAACGTATGCTTATT
   361  GGTGTTGAAAATTCTCTTGCTCAACTTGAACTTATTGATACTATTCAACGTCTTGGTATT
   421  TCTTATCGTTTTGAAAATGAAATTATTTCTATTCTTAAAGAAAAATTTACTAATAATAAT
   481  GATAATCCTAATCCTAATTATGATCTTTATGCTACTGCTCTTCAATTTCGTCTTCTTCGT
   541  CAATATGGTTTTGAAGTTCCTCAAGAAATTTTTAATAATTTTAAAAATCATAAAACTGGT
   601  GAATTTAAAGCTAATATTTCTAATGATATTATGGGTGCTCTTGGTCTTTATGAAGCTTCT
   661  TTTCATGGTAAAAAAGGTGAATCTATTCTTGAAGAAGCTCGTATTTTTACTACTAAATGT
   721  CTTAAAAAATATAAACTTATGTCTTCTTCTAATAATAATAATATGACTCTTATTTCTCTT
   781  CTTGTTAATCATGCTCTTGAAATGCCTCTTCAATGGCGTATTACTCGTTCTGAAGCTAAA
   841  TGGTTTATTGAAGAAATTTATGAACGTAAACAAGATATGAATCCTACTCTTCTTGAATTT
   901  GCTAAACTTGATTTTAATATGCTTCAATCTACTTATCAAGAAGAACTTAAAGTTCTTTCT
   961  CGTTGGTGGAAAGATTCTAAACTTGGTGAAAAACTTCCTTTTGTTCGTGATCGTCTTGTT
  1021  GAATGTTTTCTTTGGCAAGTTGGTGTTCGTTTTGAACCTCAATTTTCTTATTTTCGTATT
  1081  ATGGATACTAAACTTTATGTTCTTCTTACTATTATTGATGATATGCATGATATTTATGGT
  1141  ACTCTTGAAGAACTTCAACTTTTTACTAATGCTCTTCAACGTTGGGATCTTAAAGAACTT
  1201  GATAAACTTCCTGATTATATGAAAACTGCTTTTTATTTTACTTATAATTTTACTAATGAA
  1261  CTTGCTTTTGATGTTCTTCAAGAACATGGTTTTGTTCATATTGAATATTTTAAAAAACTT
  1321  ATGGTTGAACTTTGTAAACATCATCTTCAAGAAGCTAAATGGTTTTATTCTGGTTATAAA
  1381  CCTACTCTTCAAGAATATGTTGAAAATGGTTGGCTTTCTGTTGGTGGTCAAGTTATTCTT
  1441  ATGCATGCTTATTTTGCTTTTACTAATCCTGTTACTAAAGAAGCTCTTGAATGTCTTAAA
  1501  GATGGTCATCCTAATATTGTTCGTCATGCTTCTATTATTCTTCGTCTTGCTGATGATCTT
  1561  GGTACTCTTTCTGATGAACTTAAACGTGGTGATGTTCCTAAATCTATTCAATGTTATATG
  1621  CATGATACTGGTGCTTCTGAAGATGAAGCTCGTGAACATATTAAATATCTTATTTCTGAA
  1681  TCTTGGAAAGAAATGAATAATGAAGATGGTAATATTAATTCTTTTTTTTCTAATGAATTT
  1741  GTTCAAGTTTGTCAAAATCTTGGTCGTGCTTCTCAATTTATTTATCAATATGGTGATGGT
  1801  CATGCTTCTCAAAATAATCTTTCTAAAGAACGTGTTCTTGGTCTTATTATTACTCCTATT
  1861  CCTATG

-Loki

Attachments
limonene synthase.JPG (125 downloads)

_________________________
"Tastes like chicken..." - Ed Gein.

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#1486555 - 01/10/09 09:12 PM Re: Mothers in micro-culture [Re: Earl]
Loki Old hand Registered: 03/31/08 Posts: 1165 Loc: Laughing Skull (US)	Originally Posted By: Earl This is a list from Arjan's Strawberry Haze Are you going to be able to pick out and increase specific terpens ? . Looks like it... I've got sequences for about 3/4 of that list now, plus about 15 that aren't on that list... -Loki _________________________ "Tastes like chicken..." - Ed Gein.
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#1486596 - 01/11/09 04:37 AM Re: Mothers in micro-culture [Re: Loki]
Earl Pot Head Registered: 04/07/07 Posts: 3714 Loc: Synthetic farm	can you make some "spray on" flavor for dried weed ? _________________________ Aero Space Shuttle Current Grow: BlackJack Purple Cindy
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#1486642 - 01/11/09 09:15 AM Re: Mothers in micro-culture [Re: Earl]
Loki Old hand Registered: 03/31/08 Posts: 1165 Loc: Laughing Skull (US)	Originally Posted By: Earl can you make some "spray on" flavor for dried weed ? Well, you can buy the extracted terpenes, so you could try it and see. I'd guess it work, if you did it right... It'd probably be very easy to over do it... or not get it evenly applied... -Loki _________________________ "Tastes like chicken..." - Ed Gein.
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#1486672 - 01/11/09 10:58 AM Re: Mothers in micro-culture [Re: Loki]
GrandPoobah Bongmeister Registered: 05/28/06 Posts: 1882 Loc: next to a bong	why would you want to put anything ON your weed. Grow organic and with a variety of genes....you can adjust taste by adjusting nutes _________________________ There is no off position on the genius switch Proud member of the organic mafia
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