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#1399459 - 03/28/08 03:55 AM Mothers in microculture ****
sad old man Offline
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Registered: 03/27/08
Posts: 76
Loc: Ohio
Hello everyone. I'm new here and I'd like to pose a question to the group.
Does anyone out there know what the plant specific recipe for the agar mix used to put a cannabis cutting into in-vetro microculture is? I would like to know the amount of the hormones in each type of culture, shoot developement and rooting phase. The ammount of finished medium is to be 1 liter. Any help would be appreciated. I know I'll get some help here,so thanks in advance.
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#1399711 - 03/29/08 06:22 AM Re: Mothers in microculture [Re: sad old man]
sad old man Offline
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Registered: 03/27/08
Posts: 76
Loc: Ohio
Hey, come on. Somebody give me a comeback. At least maybe the monitor?
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#1399743 - 03/29/08 08:31 AM Re: Mothers in microculture [Re: sad old man]
Big Stinky Offline
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Registered: 11/23/07
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I would love to help but I have no idea wtf you are talking about. isn't agar the gelatinous stuff used in petri dishes for growing bacterial cultures?
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#1400065 - 03/30/08 10:45 AM Re: Mothers in microculture [Re: Big Stinky]
sad old man Offline
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Registered: 03/27/08
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check out the site planttc.com and you will know what I'm refering too.It's a way to keep your Mother plants in a culture jar that takes hardly any space, and very low wattages of light to maintain them.
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#1400066 - 03/30/08 10:46 AM Re: Mothers in microculture [Re: sad old man]
sad old man Offline
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Registered: 03/27/08
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Loc: Ohio
By the way Big Stinky, thanks for answering me back.
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#1400126 - 03/30/08 02:11 PM Re: Mothers in microculture [Re: sad old man]
Big Stinky Offline
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Registered: 11/23/07
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Wow, that is something else. Never seen anything like that, but it certainly looks like it has something going on there.

I would have no idea what hormones you would need to use for mj, but I would assume that if they have a tomato blend it would be a damn fine start for it.

If you go this route, please post and take pics! I would love to have my floorspace back in my veg room \:\)
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#1400147 - 03/30/08 03:28 PM Re: Mothers in microculture [Re: Big Stinky]
sad old man Offline
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Registered: 03/27/08
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Loc: Ohio
Ok big stinky. I'm still in the first stages of getting the required extra equipment you have to supply to get going. I'll take some photos once I get going.
Ya, my Moms are taking up valuable real estate that could be used for other things, that and the Moms seem to bring other problems into the grow. And so I'm gonna try this. If it works I'll have more than 5 Moms in culture.:)
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#1400163 - 03/30/08 05:22 PM Re: Mothers in microculture [Re: sad old man]
caymanreview Offline
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Registered: 02/04/07
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this is a good idea. ive learned a bit about microculture in school, and its a very interesting subject to me. its too bad that none of my horticulture instructors (small school, rural area) arent very knowledeable in this area for me to pick their brain.

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#1400164 - 03/30/08 05:34 PM Re: Mothers in microculture [Re: caymanreview]
Ohigho Offline

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Registered: 10/11/04
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I've heard mention of this before but not much about it.

What would the costs be for a DIY setup? I read that you have to have a completely sterile setup and have a low oxygen cooler to store them.


Edited by Ohigho (03/30/08 05:41 PM)
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#1400212 - 03/30/08 08:48 PM Re: Mothers in microculture [Re: Ohigho]
sad old man Offline
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Registered: 03/27/08
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So far I've gotten about $400.00 invested. I'm just waiting for the rest of the materials to arrive. With my indoor garden, I have a lot of the escentials already in place. Time will tell. The sterile enviroment stuff doesn't scare me off, as I've already had some experience with growing mushrooms.I haven't run into any mention of special storage of the cultures. Only as far as they need little light and kept in a clean enviroment, like my grow room, nothing extra.


Edited by sad old man (03/30/08 08:53 PM)
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