Mothers in microculture - Cannabis Culture Forums

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#1443853 - 09/07/08 06:40 PM Re: Mothers in micro-culture [Re: RRog]
Loki Old hand Registered: 03/31/08 Posts: 1165 Loc: Laughing Skull (US)	Originally Posted By: RRog Seems like if you run the risk of losing 10% due to contamination, sooner or later you'd lose strains. Contaminating can be contained to a minimum with proper procedures, keeping things isolated. You'll never get 100% sterile, but you can get close enough that you can be very productive. The more levels of containment the better. I do my cultures in petri dishes. The cells are under the agar and the agar has antibacterial additives, that's level 1... Level 2 is the dish itself. When you're not working with it, it should be sealed with gause tape. Perti dishes go in a new ziplock baggy, level 3. Level 4 is your clean box... I only open the clean box to add or remove things, and I do it through a two-step process, so I can maintain relative cleanliness... If a glove breaks on me, I throw out every dish that isn't sealed. -Loki _________________________ "Tastes like chicken..." - Ed Gein.
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#1443857 - 09/07/08 06:48 PM Re: Mothers in micro-culture [Re: Loki]
RRog Carpal Tunnel Registered: 08/26/08 Posts: 2326	OK so all cultures remain right in the box. Cool. I'd love to see the box setup whenever you have a minute. Thanks for the participation in this thread _________________________ I am not an Expert. I have so many posts because I ask a lot of questions.
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#1449441 - 09/21/08 08:52 PM Re: Mothers in microculture [Re: sad old man]
goose37 Stranger Registered: 01/09/06 Posts: 12	the starter kit,.. http://www.hahnslighting.com/?p=64 take care , goose
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#1449505 - 09/22/08 11:22 AM Re: Mothers in microculture [Re: goose37]
RRog Carpal Tunnel Registered: 08/26/08 Posts: 2326	Thanks Goose _________________________ I am not an Expert. I have so many posts because I ask a lot of questions.
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#1455042 - 10/08/08 08:36 AM Re: Mothers in microculture [Re: RRog]
Phantom Blooper Journeyman Registered: 09/27/08 Posts: 54	wow, this is the coolest thing I have ever seen. Has anyone posting in this thread generated plants from this method and had them leave the tube?
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#1468918 - 11/22/08 12:57 AM Re: Mothers in micro-culture [Re: sad old man]
Loki Old hand Registered: 03/31/08 Posts: 1165 Loc: Laughing Skull (US)	Originally Posted By: sad old man Thanks for the support, I'll keep trying to get this right.It takes time for things to evolve and it's slow work. I'm trying to do this without making too many mistakes along the way. Before I do any more transfers though I'm going to need to do some improvements in my hood. I don't want to lose the cultures I've got going so far. I've already had to start over once. SOM, You still around, bud? I've got my new gear up and running... wanted to share some DNA... -Loki _________________________ "Tastes like chicken..." - Ed Gein.
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#1469043 - 11/22/08 09:24 AM Re: Mothers in micro-culture [Re: Loki]
tin0 Stoner Registered: 07/30/06 Posts: 426	Nice setup Loki! Looks like youve got contaminations taken care of. haha
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#1469187 - 11/22/08 03:57 PM Re: Mothers in micro-culture [Re: tin0]
Earl Pot Head Registered: 04/07/07 Posts: 3714 Loc: Synthetic farm	Nice box Loki. I'm watching to see if you guys can get those cultures to grow back into flower producing plants again. I'm sure you guys can do it, I just like watching you. Thanks for the pictures. _________________________ Aero Space Shuttle Current Grow: BlackJack Purple Cindy
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#1469254 - 11/22/08 06:45 PM Re: Mothers in micro-culture [Re: Earl]
RRog Carpal Tunnel Registered: 08/26/08 Posts: 2326	That's super, Loki. You'll go a long way with that. I've never used one. _________________________ I am not an Expert. I have so many posts because I ask a lot of questions.
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#1469282 - 11/22/08 08:08 PM Re: Mothers in micro-culture [Re: Loki]
GrandPoobah Bongmeister Registered: 05/28/06 Posts: 1882 Loc: next to a bong	I hope it works, would be way easier to send genes than fedexing fresh clones _________________________ There is no off position on the genius switch Proud member of the organic mafia
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