That's some starts of AK48 and pure power plant. This is a closer view of the media and the cutting. x-plant.
The date of start was 4/14/8. I'll update photos as I see any changes.

I hope this goes smoothly for you. I am keenly interested in this method, and I hope you have good luck with it so we can see a grow from start to finish.

I think I can speak for most of the grow nerds here, that not only are pictures great to see, but detailed descriptions of what, where, when, why and how :), especially for something as far from mainstream as this is.

Thought I would let you know that I've been growing all my life and have had a grow going indoors for 7 years running. The mothers I've kept are getting old and I've been looking for a way to save some space. I fly hydro. Areo-flo 20's with 30 day spacing between harvests. 1, 12/12 room with the 2,Areo-flo 20's and 2, 1000 w. lights on movers and 1 large closet for moms and the upcoming veging crop, along with the cloning area, all under a 2x4 fixture I put together with 8, T8 lamps. Really, it's about saving space and getting new plants that are in perfect health.

I'm going to try something similar. I have a Jack Herer that's flowering now and it's definately some DNA I want to grow again.

What are you using for agar? I ordered 2 standarded mixes, as well as hormones to root/shout/split. And a vitamin and anti-botic additives... I've got just under $100 in those mats, but should be enough to last years...

I also picked up a USB microscope. I want to do some time lapse video of the cells dividing. And I've been doing some reading on Chimerical plants from tissue cultures and it seems like it'd be fairly easy to try with pot. I've started a few potental cross tissue donors from some different types of plants.

One thing I found that seemed very interesting was a white paper on the thornless blueberry. The L1 cell layer of a regular blueberry was replaced with the L1 cells from another type of plant. What if it was pot L1 cells? Would it produce THC trichomes when it flowered? Anyway, that's what I'm hoping for, a Chimeric pot plant that looks like, well, anything but a pot plant. (My friends think I'm nuts and ask me when the brocolli hash is gonna be ready)

-Loki

Loki...... I'm working from a pre measured kit of materials. These are the materials included in the medium mixture reciepe,A nut mix containing hydro ferts along with some added vitamins. The jelling agent is Gellim Gum Gel Powder. Also added into the medium is a multiplication pack of hormones and an added preservitive.
It's hard to tell what you could cross together since we're working on the genetic level. I'm just hoping to save some space in my room.LOL

Well, it's been 4 days and I can't see any contamination in any of the tubes yet. Good sign. This attached photo is of a PPP start and it's showing some growth already.

This is a shot of an AK x-plant with some shoots starting.

Nice... but are you going to start a 'mother culture' or just make clones from explants? So you can keep your mother plant tiny and still make lots of clones?

I originally only wanted to get a clone from a plant that has nearly finished flowering, but now I'm thinking there might be so much more possible. I've been reading the pot forums and haven't seen very much about microbiotic pot experimentation though. I've actually been looking into extracting DNA and then inserting it into other cells. There are actually online labs for extracting DNA from frozen peas that could possibly be ported to pot... and the first gene gun was made from a crossman air pistol... I've even got one of those!

This is kind of off topic, but it does relate to space issues, which you've said is a factor which lead you to this... but what kind of lights do you use? I know a lot of people who think they're cutting edge with HPS and CFL, but if you haven't tried LEDs... you just don't know what you're missing. They emit so little heat you lose very little water to evaporation and you basicly have little terriariums. You could easily do big plants, but I prefer to grow 8 shorties rather than 1 tall girl, you can shave a few weeks of veg time and get the same yield...

-Loki

Loki,
It's all tied together. Use Mother's in culture to generate shoots for new clones, while maintaining the Mother. It should take 1 month to generate roots and to condition the new plants into the regular enviroment, hydro/soil, then slip them into 12/12 to flower. If you had 4 tubes of each of the mother plants, you could have 9 mothers of diffrent strains in a space of only 8"x12".total of 36 tubes. Once a month you have to rejar or retube the moms to maintain them. I probably confused the shit out of you. Sorry if I did.

Okay, that makes sense, so your mother plant agar is mixed to produce shoots, which you transfer to a rooting agar to make clones? And then you can just discard any extra shoots to keep the mother plant in her test tube, right?

I'm going to try and keep the mother plant as just a tissue culture, no shoots or roots. So I can try and mix cells from different cultures and then root/shoot/clone the mixed culture.

-Loki