Loki, Yep you got it right. All that's required to keep the Mom's happy is to re-tube with fresh medium every 30 days or so.
If your not gonna use a mom for a specific cycle just cut off a fresh shoot from mom and re-tube,trash what's not needed, she's good to go and be maintained for another cycle. No worry about generation decline, since it's the same genetics.

How fresh of a sample do you need, to culture some cells?

Can it be from a day old cutting?

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Well, the fresher the better. I'd think a day or two would be ok, depending how the cutting was handled. If you put it in some water, like a vase, and kept in a cool area, you'd be able to get viable cells for at least a week or more. Technically, you only need 1 cell. I've gotten good onion cells after the onion was cut in half and stored in the fridge for at least a week or more.

-Loki

Hey Sad Old Man,
want some Purple Cindy cells,
before I chop her down?

Thanks for the offer Earl, but I seem to have enough for now. She looks pretty tempting in your photo. Speaking of photo, here is a shot of the PPP at 8 days into this. I'm not seeing any contamination as of yet and I think she's starting to grow some.

This is a shot of the AK at 8 days. Of all of the cultures I took during this first attempt. 16 of the pot starts, 8 African Violet, 1 Fern, and dividing some Iris, 12 of those, I don't see anything bad growing in any of the tubes or jars. Just lucky I guess. I would of thought that by now any contamination would have shown up. Just waiting and watching things grow.

Hey Earl, Have you ever ordered seeds from highgrade seeds and gotten them? I was looking to pick up some blueberry beans.

Yes, I am growing some Purple Cindy from HGS right now.

Here is a link for ya.
Purple Cindy Grow Log

I have received two packages from HGS,
and they guarantee delivery.

So if they don't show up in a week or two,
politely let them know,
and they will send replacements.

My first order was when they were taking credit cards.
I ordered online Sunday, and I got the seeds on Wednesday.

But they don't take credit cards anymore,
because of the US government.

They are a good company to deal with.

Be sure and enter the HGS Seed contest.

Good luck.

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Thanks Earl, I'll give them a try. I've been wanting to get some Blueberry going again. I love how long the buzz lasts and it takes the pain down a notch for a good long time. I let my Mother go a couple of years ago and haven't had any since.
I'm wanting to get that and Northern Lights in culture, along with the White Widow, AK, and PPP that I already have. I let my N. L. Mom go a couple of years ago also and I want her back now I can keep more strains going.
I've been out of town for a couple of days and haven't taken a look at the micro grow for a few days so there might be some pics. in the next post.

I hope you can get this technique perfected.

Seems like a good way to save genetics.

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